PP112 - SPROUTING ENHANCES DIGESTIBILITY, INTESTINAL AMINO ACID TRANSPORTER EXPRESSION AND ANTIOXIDANT POTENTIAL OF PLANT-BASED PROTEIN MATRICES
PP112
SPROUTING ENHANCES DIGESTIBILITY, INTESTINAL AMINO ACID TRANSPORTER EXPRESSION AND ANTIOXIDANT POTENTIAL OF PLANT-BASED PROTEIN MATRICES
C. BOMPART1,*, S. ROUGE1, S. MICHEL 1, M.-L. COLLIN 1, C. GIRAUDET 1, J. SALLES 1, S. WALRAND1,2, F. CAPEL1, A. ROSSARY1, M. GUEUGNEAU1
1Université Clermont Auvergne, INRAE, UNH, 63000 , 2Department of Clinical Nutrition, Clermont-Ferrand University Hospital, Clermont-Ferrand, France
Rationale: Seed sprouting is a bioprocess that may improve the nutritional quality of plant proteins while maximizing the health benefits of their matrix.
Methods: Two plant-based matrices prepared from wheat and lentils flours, both sprouted and unsprouted were compared to an animal matrix (casein). These matrices were subjected to in vitro digestion according to the INFOGEST protocol. Monolayers of intestinal cells derived from caco-2/HT29-MTX co-culture were incubated for 4 hours with the digests. The protein expression of amino acid transporters in intestinal cells was analyzed by Western-Blot. Blood leukocytes were incubated for 2 hours with the digests to assess their oxidative potential. Results were analyzed by one-way ANOVA and expressed as mean ± sem.
Results: Sprouting increased the degree of protein hydrolysis at the end of in vitro digestion compared with the unsprouted plant matrix (p=0.0397). It also led to a significant increase in PEPT1 protein expression compared to the casein matrix (p=0.0180) and restored the expression of the ATB0,+ and SNAT1 transporter proteins. These were expressed at lower levels after treatment with the unsprouted matrix compared to casein (p=0.0089, p=0.0208). Finally, digests from plant matrices exerted an antioxidant effect on blood leukocytes, regardless of sprouting status (p<0.0001).
Conclusion: These results suggest that sprouting improves the nutritional quality of plant proteins by increasing their digestibility and enhancing PEPT1 expression, a di- and tripeptide transporter, which suggests the presence of bioactive peptides generated during sprouting. By restoring the expression of certain transporters, sprouting may improve the intestinal absorption of plant proteins. Finally, these findings highlight differences in the regulation of amino acid transporters in intestinal cells between plant- and animal-based matrices.
Disclosure of Interest: None declared