Sep 14, 2025
1:00 PM2:00 PM
Poster Session
P438
EFFECT OF 1000 ΜG AND 2000 ΜG VITAMIN B12 SUPPLEMENTATION ON NEUROPATHIC PARAMETERS IN PATIENTS WITH DIABETIC PERIPHERAL NEUROPATHY AND LOW VITAMIN B12 LEVELS
A. Mansour1, S. M. Sajjadi-Jazi1,2,*, A. H. Esmaeili3, H. Gerami4, A. S. Khorasanian5, H. R. Fateh6, A. Amrollahi Bioky1, H. R. Aghaei Meybodi1, M. R. Mohajeri-Tehrani1, R. Safyari7
1Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, 2Department of Endocrinology, Shariati Hospital, School of Medicine, Tehran University of Medical Sciences, 3Department of Nutrition, Science and Research Branch, Islamic Azad University, Tehran, 4Nutrition and Food Security Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, 5Department of Nutrition, School of Public Health, Iran University of Medical Sciences, 6Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, 7Department of Internal Medicine, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran, Islamic Republic Of
Rationale: This study aimed to evaluate the effects of two different dosages of vitamin B12 supplementation on neuropathic symptoms in patients with low vitamin B12 levels.
Methods: This randomized, double-blind, placebo-controlled trial was conducted at a diabetes clinic. Diagnosis was based on at least one of the following criteria: Michigan Neuropathy Screening Instrument Examination (MNSIE) score ≥2.5 or Neuropathy Disability Score (NDS) ≥3. Sural nerve conduction studies were used for further confirmation. Among the total of 132 patients with DPN, 35 patients with low vitamin B12 levels (<200 pg/ml) and normal folate levels (>3.0 ng/mL) were included. Participants were randomized to receive either 1000 or 2000 μg of vitamin B12 daily for 16 weeks. Due to dropouts, 32 participants completed the study. Statistical analysis was performed using ANCOVA for between-group and paired t-tests or Wilcoxon tests for within-group comparisons.
Results: The mean age was 57.88±8.54 years, mean diabetes duration was 14.59±9.13 years, and mean baseline HbA1c was 7.53±0.94%. In both the 1000 μg (n=16) and 2000 μg (n=16) vitamin B12 groups, there was a significant increase in vitamin B12 levels from baseline to 16 weeks, with median values rising from 168.50 to 664.50 pg/ml (P<0.001) in the 1000 μg group, and from 167.00 to 901.50 pg/ml (P=0.001) in the 2000 μg group. Both groups showed significant improvements in the Numeric Rating Scale (NRS) (1000 μg: p=0.016, 2000 μg: p=0.007) and MNSIE scores (1000 μg: p=0.033, 2000 μg: p=0.022). However, no significant differences were observed between the two dosage groups in neuropathic outcomes (p >0.05).
Image:
Conclusion: Vitamin B12 supplementation at both 1000 μg and 2000 μg doses significantly improved neuropathic symptoms in patients with DPN and low vitamin B12 levels, but there was no advantage of a higher dose.
Disclosure of Interest: None declared