PD576 - TARGETED ANTI-INFLAMMATORY NUTRITIONAL THERAPY COMBINED WITH ENERGY RESTRICTION REPROGRAMS SPHINGOLIPID METABOLISM AND IMPROVES HEALTH IN OBESE MICE

PD576

TARGETED ANTI-INFLAMMATORY NUTRITIONAL THERAPY COMBINED WITH ENERGY RESTRICTION REPROGRAMS SPHINGOLIPID METABOLISM AND IMPROVES HEALTH IN OBESE MICE

M. Sun^1,*, W. Chen², T. Zheng¹, F. Xu²

¹ Fuzhou University Affiliated Provincial Hospital, Fuzhou, ²Peking Union Medical College Hospital, Beijing, China

Rationale: Obesity-associated metabolic disorders remain a global health challenge, and energy-restricted dietary interventions alone often yield limited benefits. In this study, we aimed to investigate the metabolic effects and underlying mechanisms of an anti-inflammatory dietary formula comprising n-3 fatty acids, chlorogenic acid, and epigallocatechin gallate. 

Methods: The formula was administered alone or in combination with an energy-restricted diet in high-fat diet–induced obese mice. Male C57BL/6 mice were subjected to an 8-week intervention, during which body weight, body composition, glucose and lipid metabolism, inflammatory markers, and tissue histopathology were monitored.

Results: The combined intervention produced greater improvements in adiposity, hepatic lipid accumulation, glucose tolerance, and systemic inflammation than either strategy alone. Non-targeted metabolomics revealed substantial reversal of high-fat diet–induced metabolic disturbances, with sphingolipid metabolism identified as a key regulated pathway. Targeted validation confirmed regulation of pathway-related metabolites and downregulation of sphingolipid synthase genes.

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Conclusion: These findings support combined anti-inflammatory and energy-restricted dietary strategies for obesity management.

Disclosure of Interest: None declared