PD614 - EVALUATION OF GLUCOSE HOMEOSTASIS IN MICE FED STANDARD AND HIGH-FAT DIETS SUPPLEMENTED WITH AMMONIUM CHLORIDE

PD614

EVALUATION OF GLUCOSE HOMEOSTASIS IN MICE FED STANDARD AND HIGH-FAT DIETS SUPPLEMENTED WITH AMMONIUM CHLORIDE

B. L. C. Ferreira^1,*, M. E. O. Araújo¹, S. Andreotti¹, C. R. O. Carvalho¹

¹Physiology and Biophysics, University of São Paulo, São Paulo, Brazil

Rationale: Metabolic acidosis has been associated with alterations in glucose homeostasis. Ammonium chloride (NH₄Cl) induces metabolic acidosis, while high-fat diets (HFD) promote glucose intolerance. However, their combined effects on glucose metabolism remain unclear.

Methods: 7-week-old male C57BL/6J mice were acclimatized for 12 days and randomly assigned to four groups: standard diet (S), standard diet + NH₄Cl (S+), HFD, and HFD + NH₄Cl (HFD+), receiving 1% NH₄Cl for 10 days. Glucose and insulin tolerance tests (oGTT: 2g/kg; ipITT: 0.5mU/g) were performed. Glycemia was measured over time, and AUC was calculated. Data were analyzed using two-way ANOVA with Tukey’s post hoc test. CEUA USP 4584210825.

Results: oGTT show that HFD-fed mice exhibited higher glycemia over time. AUC analysis revealed significant effects of diet (p < 0.0001) and NH₄Cl (p = 0.0381), without interaction (p = 0.2154). In the ipITT, no interaction was observed (p = 0.5947), and NH₄Cl had no effect (p = 0.5069), while diet remained significant (p < 0.0001). Fasting glycemia (6h) showed a diet effect (p = 0.0002), with no effect of NH₄Cl (p = 0.9414) or interaction (p = 0.3552).

Conclusion: Short-term HFD impaired glucose tolerance and insulin sensitivity regardless of NH₄Cl supplementation.

Disclosure of Interest: None declared