P1008 - OXIDATIVE-ANTIOXIDATIVE STATUS IN CHILDREN WITH CELIAC DISEASE.

P1008

OXIDATIVE-ANTIOXIDATIVE STATUS IN CHILDREN WITH CELIAC DISEASE.

G. Rowicka^1,*, J. Ambroszkiewicz², W. Klemarczyk³, H. Dyląg³, A. Liber³, M. Chełchowska²

¹Gastroenterology Outpatients Clinic, ²Department of Screening Tests and Metabolic Diagnostics, ³Department of Nutrition, Institute of Mother and Child, Warsaw, Poland

Rationale: Celiac disease (CD) is a chronic, immune-mediated small intestinal enteropathy, caused by wheat gluten and related proteins from other grains. Oxidative stress is a factor involved in the pathogenesis of CD, potentially influencing disease progression and celiac-related complications. We assessed the serum levels of oxidative and antioxidative markers in children with newly diagnosed CD, both at the time of diagnosis and after six months of a gluten-free diet (GFD).

Methods: The study included 16 children with newly diagnosed CD. Serum concentrations of total oxidative capacity (TOC) and oxidized glutathione (GSSG) were analyzed as markers of oxidative stress, while total antioxidative capacity (TAC) and reduced glutathione (GSH) were measured as markers of antioxidative defense. The oxidative stress index (OSI) was calculated as the TOC/TAC ratio, and the GSH/GSSG ratio (R-index) indicating the cellular redox state, was also determined. All biochemical analyses were conducted using enzyme-linked immunosorbent assays (ELISA).

Results: In children with newly diagnosed CD no significant differences were observed in the concentrations of oxidative markers (TOC, GSSG) or antioxidative markers (TAC, GSH), as well as in OSI and R-index values, after six months of adherence to a gluten-free diet (p > 0.05). However, TOC and OSI levels decreased by 25% and 30%, respectively, while GSH concentrations and the R-index increased by approximately 20% following six months of dietary treatment.

Conclusion: The findings suggest that six months of a gluten-free diet may not be sufficient to achieve significant improvements in the oxidative-antioxidative balance in children with celiac disease. The relatively small study group may also be a limiting factor, therefore, further research with a larger cohort is ongoing.

Disclosure of Interest: None declared