P169 - EFFICACY OF HYDROLYZED CONCENTRATE AND HEME IRON-FORTIFIED FLOUR IN A PHENYLHYDRAZINE-INDUCED ANEMIA RAT MODEL

P169

EFFICACY OF HYDROLYZED CONCENTRATE AND HEME IRON-FORTIFIED FLOUR IN A PHENYLHYDRAZINE-INDUCED ANEMIA RAT MODEL

J. M. Giorgi Ortiz^1,*, V. Sánchez Torres¹, N. Moreno Castellanos¹

¹Santander, Universidad Industrial de Santander, Bucaramanga, Colombia

Rationale: Anemia is a major global health problem, caused mainly by iron deficiency. This study evaluates the effects of a hydrolyzed bovine blood concentrate and a heme iron-fortified flour on biochemical, hematological, and physiological parameters in a phenylhydrazine (PHZ)-induced anemia model in rats.

Methods: Male Wistar rats were divided into six groups: normal control (NC), anemic control (AC), normal + hydrolysed concentrate (NHC), anemia + hydrolysed concentrate (AHC), normal + haem iron-enriched flour (NHF) and anemia + haem iron-enriched flour (AHF). Anemia was induced using PHZ (40 mg/kg i.p.), and the treatments were administered for 20 days. Biochemical and hematological parameters were analyzed, organ weights were recorded, and histological analyses of the liver and spleen were performed.

Results: The anemic groups showed significant reductions in red blood cell count, hemoglobin and hematocrit compared to the control group. Both the hydrolyzed concentrate and the heme iron-enriched flour significantly improved these parameters, restoring values similar to those of the control group. The NHF and AHF groups showed hematological responses similar to those of NHC and AHC, but the animals in the groups fed with flour had a higher final body weight. Organ weights and histological characteristics were consistent in all supplemented groups, with spleen hypertrophy and liver congestion observed in the anemic controls, but reduced in the treated groups. The results confirm that both hydrolyzed concentrate and flour fortified with heme iron effectively improve parameters related to anemia, and that flour provides additional benefits in terms of weight gain. 

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Conclusion: Flour fortified with heme iron presents a promising alternative to hydrolyzed concentrate for the treatment of anemia, offering comparable hematological benefits with superior results in terms of weight gain.

Disclosure of Interest: None declared