PP106 - EFFECTS OF THE ADMINISTRATION OF PROBIOTIC COMPLEXES OF BACILLUS COAGULANS AND CLOSTRIDIUM CASEI ON INTESTINAL BARRIER DAMAGE INDUCED BY 5-FLUOROURACIL IN RATS

PP106

EFFECTS OF THE ADMINISTRATION OF PROBIOTIC COMPLEXES OF BACILLUS COAGULANS AND CLOSTRIDIUM CASEI ON INTESTINAL BARRIER DAMAGE INDUCED BY 5-FLUOROURACIL IN RATS

S. Cao^1,*, Y. Zhou¹, Y. Tian¹

¹the affiliated hospital of qingdao university, Qingdao, China

Rationale: Intestinal barrier injury is widespread after treatment with antitumour drugs such as 5-fluorouracil (5-FU), but there is still a lack of prevention and effective management strategies based on fundamental research evidence.

Methods: An intestinal barrier injury model was established in male SD rats, aged 2 months, with a body weight ranging from 240 g to 260 g. via the intraperitoneal injection of 5-FU (70 mg/kg/d), and the probiotic complex (1×109 CFU/ml or 1×108 CFU/ml) was administered via gavage. The body weight, food intake and diarrhoea of the animals were monitored, euthanasia on the 9th day.Male Sprague-Dawley (SD) rats at 2 months of age and weighing 240 to 260 grams were selected. The animals were divided into an experimental group and a control group, with 6 rats in each group.

Results: Compared with the control group, the probiotic complex intervention increased the food intake (P < 0.05) of the experimental animals, delayed weight loss (P< 0.05), alleviated diarrhoea (P< 0.05), and improved the histopathological score of ileal mucosal injury (P< 0.05). The probiotic complex  intervention decreased the levels of IL-1β (P < 0.05), IL-6 (P < 0.001), MPO (P < 0.001), TNF-α (P < 0.001), and LPS (P< 0.001) in the jejunum, whereas the level of the anti-inflammatory factor IL-10 increased (P< 0.05). Furthermore, there was a significant increase in butyrate-producing bacteria, including Lactobacillus, Acidophilus, Clostridium, UCG-014, Muridae, and Lachnospiraceae NK4A136, in the intervention group.

Conclusion: Administration of probiotic complexes of Bacillus coagulans and Clostridium casei has the ability to improve intestinal mechanical, chemical, immune, and biological barrier functions and plays a protective role against 5-FU-induced intestinal barrier damage in rats.

Disclosure of Interest: None declared