P847 - CHANGES IN ANTHROPOMETRIC, BIOCHEMICAL, INFLAMMATION AND AGING MARKERS AND VITAMIN D IN OVERWEIGHT AND OBESE PERSONS BEFORE AND AFTER A 3-MONTH DIETARY INTERVENTION: A PILOT STUDY

P847

CHANGES IN ANTHROPOMETRIC, BIOCHEMICAL, INFLAMMATION AND AGING MARKERS AND VITAMIN D IN OVERWEIGHT AND OBESE PERSONS BEFORE AND AFTER A 3-MONTH DIETARY INTERVENTION: A PILOT STUDY

D. Holmannova^1,*, T. Soukup¹, T. Svadlakova^1,2, C. Andrys², P. Borsky¹, V. Blaha³, M. Esterkova¹, L. Borska¹

¹Department of Preventive Medicine, Faculty of Medicine in Hradec Kralove, ²Department of Clinical Immunology and Allergology, ³The 3rd Department of Internal Medicine – Metabolic Care and Gerontology, University Hospital Hradec Kralove and Faculty of Medicine in Hradec Kralove , Hradec Kralove, Czech Republic

Rationale: Overweight and obesity are major health issues with high morbidity and mortality. This pilot study assessed the effects of a 3-month dietary intervention on various health markers to evaluate interindividual variability and support personalized approaches in metabolic diseases.

Methods: Thirty-seven overweight/obese adults (grouped by sex, age, baseline BMI, medication use) underwent a 3-month reduced-energy diet, higher protein intake with an 11-hour eating window. We measured anthropometric data (BMI, waist circumference, fat %, visceral fat, fat volume), blood pressure, biochemical markers (glycemia, cholesterol fractions, triglycerides), adipokines, inflammation and aging markers (CRP, IL-6, sCD40L, LL-37, MCP-1, leptin, adiponectin, NAD, telomerase, AGEs), and vitamin D before and after the intervention. Parametric and non-parametric tests and Spearman rank correlation was used. 

Results: The intervention improved anthropometric parameters and reduced leptin levels across all participants (p<0.01 or p<0.001). Outcomes varied by sex, age, BMI, and medication. Women had higher HDL, leptin, vitamin D (p<0.01/0.05, p<0.001, p<0.01/0.05) and men had higher adiponectin and LL-37 (p<0.001, p<0.01/0.05) before/after intervention. Lipids decreased mainly in men, participants with BMI >35, and those <50 years (p<0.01 or/and p<0.05). Glycemia improved in women and those >50 years (both p<0.05). sCD40L declined in men with BMI >35 and women with BMI <35 (both p<0.05); IL-6 decreased in women (p<0.05). Leptin correlated with anthropometric parameters and CRP and AGEs; post-intervention IL-6 correlated with anthropometric data.

Conclusion: Dietary intervention improved key markers, but responses differed by sex, age, BMI, and treatment. Personalized dietary strategies appear crucial for effective management of metabolic conditions.

Disclosure of Interest: None declared