LB047 - TGF-Β2 DETECTION IN A SEMI-ELEMENTAL FORMULA FOR NUTRITIONAL SUPPORT IN MALABSORPTION
LB047
TGF-Β2 DETECTION IN A SEMI-ELEMENTAL FORMULA FOR NUTRITIONAL SUPPORT IN MALABSORPTION
G. Oliveira1,*, J. L. Roberto2, A. P. Biz2, C. M. Pereira1, A. C. Zanini1, A. P. M. Celes3, H. Santos1
1Scientific Department, 2R&D Department, 3Technical Director and Co-founder of Prodiet Medical Nutrition, Prodiet Medical Nutrition, Curitiba, Brazil
Rationale: The presence of TGF-β2 in nutritional formulas may offer immunomodulatory and regenerative benefits for patients with gastrointestinal disorders such as malabsorption. This study aims to quantify the concentration of TGF-β2 in a semi-elemental formula based on hydrolyzed whey protein.
Methods: The quantification of bovine TGF-β2 was performed using a sandwich-type ELISA assay. The methodology was based on the immobilization of anti-TGF-β2 antibodies, followed by the addition of samples and biotinylated detection antibodies. After incubation, washing, and addition of HRP-Streptavidin, the reaction was visualized with TMB substrate and stopped with a stop solution. The absorbance was read at 450 nm, and the concentration was determined using a standard curve fitted with a four-parameter logistic (4PL) model, with R² = 0.992. Samples of the formula (Peptimax – Prodiet Medical Nutrition) were analyzed under two conditions: (1) supernatant without dialysis and (2) dialyzed with a <3.5 kDa cutoff membrane to remove low molecular weight interferents.
Results: The concentration of TGF-β2 in the analyzed formula increased from 61.12 pg/mL to 87.70 pg/mL after dialysis with a <3.5 kDa cutoff membrane. This increase indicates that the original matrix contained low molecular weight interferents that inhibited cytokine detection. The removal of these compounds improved assay sensitivity, suggesting that a fraction of TGF-β2 was partially masked.
Conclusion: The semi-elemental formula contains detectable levels of TGF-β2, with concentrations varying according to sample processing methods. Notably, dialysis using a <3.5 kDa membrane increased the measured concentration, underscoring the critical role of sample preparation in accurate quantification. Given the known ability of TGF-β2 to mitigate chemotherapy-induced diarrhoea and mucositis, its presence in Peptimax suggests potential protective benefits for intestinal health.
References: Wang M.Y. et al. The Research Progress in Transforming Growth Factor-β2. Cells. 2023; 12(23):2739.
Nguyen D.N. et al (2015). Protective effects of transforming growth factor β2 in intestinal epithelial cells by regulation of proteins associated with stress and endotoxin responses. PLOS ONE, 10(2).
Antoun S. et al. (2009). Protective effect of an enteral formula containing TGF-β2 in the prevention of chemotherapy-induced diarrhoea: A pilot study. E-Spen,The European E-Journal of Clinical Nutrition and Metabolism, 4(6).
Silva F.L. Efeito do processamento na concentração do fator de crescimento TGF beta em lácteos / Fernanda Lopes da Silva. – Viçosa, MG, 2015.
Disclosure of Interest: G. Oliveira Other: Prodiet Medical Nutrition employee, J. Roberto Other: Prodiet Medical Nutrition employee, A. Biz Other: Prodiet Medical Nutrition employee, C. Pereira Other: Prodiet Medical Nutrition employee, A. Zanini Other: Prodiet Medical Nutrition employee, A. P. Celes Other: Prodiet Medical Nutrition employee, H. Santos Other: Prodiet Medical Nutrition employee