Sep 15, 2025
1:00 PM2:00 PM
Poster Session
P1061
PARENTERAL SUPPLEMENTATION OF BETA-HYDROXY-BETA-METHYLBUTYRATE (HMB) REDUCES SUBCUTANEOUS FAT WEIGHT IN MICE WITHOUT MODULATION OF AMPK/AKT/MTOR SIGNALING PATHWAY
M. Noguchi1,*, H. Takayama1,2, T. Narita3,4, R. Inoue1, N. Matsumoto4, S. Tsuihiji1, S. Murakoshi1,5, K. Fukatsu1,4
1The University of Tokyo Hospital, Surgical Center, 2St. Luke's International Hospital, Department of Nutrition, 3The University of Tokyo Hospital, Gastrointestinal Surgery, 4The University of Tokyo, Operating Room Management and Surgical Metabolism, Tokyo, 5School of Nutrition & Dietetics, Faculty of Health & Social Work, Kanagawa, Japan
Rationale: We have demonstrated HMB-supplemented parenteral nutrition (HMB-PN) atrophy of gut associated lymphoid tissue (GALT), prevent gut atrophy, and decrease subcutaneous (S.C.) fat weight without changing skeletal muscle or visceral fat weight. Though increased mTOR expression is associated with GALT recovery, precise mechanism underlying decreased S.C. fat weight remains unclear. Here, we investigated influences of HMB-PN on AMPK/Akt/mTOR signaling pathway in subcutaneous fat, which is deeply associated with fat metabolism.
Methods: Six weeks old male Institute of Cancer Research mice (n=16) were randomly assigned to Control (n=8) or HMB (n=8) group. Mice were inserted a catheter into the right jugular vein and were continuously administered PN solution for 5 days. Ca-HMB (2000mg/kg/day BW) was added in the TPN solution of the HMB group. After the dietary manipulation, all mice were killed and right calf muscle, subcutaneous fat, and visceral fat were isolated and weighed. Total cell lysate was prepared from subcutaneous fat tissue, and applied for western blot analysis. Mann-Whitney U-test was used for all statistical analyses.
Results: We confirmed HMB-PN to reduce S.C. fat weight but not visceral fat weight, compared to Control PN as in our previous study. Western blot analysis revealed that expression of AMPK, Akt or mTOR was not changed by 5 days HMB-PN. In addition, phosphorylation of AMPK, Akt, or mTOR was not increased.
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Conclusion: HMB-PN reduces S.C. fat weight without activating AMPK / Akt / mTOR pathway.
References: -
Disclosure of Interest: None declared