O078 - AMMONIUM CHLORIDE MODULATES BODY COMPOSITION IN MICE FED STANDARD AND HIGH-FAT DIETS
O078
AMMONIUM CHLORIDE MODULATES BODY COMPOSITION IN MICE FED STANDARD AND HIGH-FAT DIETS
M. E. O. Araújo1,*, B. L. C. Ferreira1, S. Andreotti1, C. R. O. Carvalho 1
1 Physiology and Biophysics, University of São Paulo , São Paulo , Brazil
Rationale: The metabolic effects of dietary acid load remain poorly understood, particularly in the context of obesity. Understanding how mild dietary acidification influences body composition may provide insights into metabolic adaptations associated with obesity and diet-induced metabolic alterations.
Methods: Male C57BL/6J mice (7 weeks old) were acclimatized for 12 days and then assigned to four experimental groups for 10 days: (1) standard diet AIN-93M (S); (2) AIN-93M supplemented with 1% NH₄Cl (S+); (3) high-fat diet (57% fat) (HFD); and (4) HFD supplemented with 1% NH₄Cl (HFD+). Body weight and food intake were recorded daily. Body composition (fat and lean mass) was assessed by nuclear magnetic resonance. Data were analyzed using two-way ANOVA followed by Tukey’s post hoc test. CEUA USP 4584210825.
Results: NH₄Cl supplementation did not affect food intake in either dietary group. As expected, mice fed a HFD showed higher caloric intake than those fed the standard diet (p < 0.0001). Body weight gain during the experimental period was not altered by NH₄Cl supplementation. However, analysis of final body weight revealed that mice fed HFD+ exhibited lower body weight compared with non-supplemented HFD animals (p < 0.05). Regarding body composition, HFD-fed mice showed increased adiposity compared with controls (p < 0.0001). Notably, NH₄Cl supplementation significantly reduced fat mass and relative fat mass in HFD-fed mice (p < 0.05). In parallel, NH₄Cl supplementation increased the relative proportion of lean mass (p < 0.05).
Conclusion: NH₄Cl supplementation altered body composition in mice fed a high-fat diet, reducing adiposity and increasing the relative proportion of lean mass without affecting food intake.These results indicate that mild dietary acid load modulates body composition under high-fat diet conditions.
Disclosure of Interest: None declared