P922 - LONG-TERM CHANGES IN GUT MICROBIOTA DIVERSITY AND FECAL METABOLITES IN PATIENTS RECEIVING ENTERAL NUTRITION

P922

LONG-TERM CHANGES IN GUT MICROBIOTA DIVERSITY AND FECAL METABOLITES IN PATIENTS RECEIVING ENTERAL NUTRITION

A. Ito^1,*, Y. Kobayashi², C. Yamamoto³, K. Arai⁴, H. Sonoki¹

¹Health Care & Nutritional Science Institute, Morinaga Milk Industry, Zama, ²Department of Surgery, ³Department of Nutrition, ⁴Department of Clinical Laboratory, Sakura General Hospital, Oguchi, Aichi, Japan

Rationale: Recent studies have highlighted the importance of gut microbiota in various diseases, yet its status in enteral nutrition patients remains unclear. This study aims to investigate the impact of enteral nutrition on gut microbiota and its clinical significance by observing the diversity of gut microbiota and fecal metabolites in enteral nutrition patients over the long term.

Methods: The study involved patients receiving enteral nutrition with fiber-enriched liquid diets. 9 cases (68±15 years old; 5 men, 4 women) that could be tracked from 2021 to 2022 were analyzed for gut microbiota, organic acids, and putrefactive products in fecal samples.

Results: The follow-up period was 120±31 days, and the diversity index of gut microbiota remained stable (4.8±0.4 to 4.9±0.4). The change in the diversity index (range: -0.6 to 0.5) showed a positive correlation trend with the change in Actinobacteria (r=0.605, p=0.084). When divided into two groups based on the increase (positive group) or decrease (negative group) of Actinobacteria, the positive group showed an increase in acetic acid (3.1±1.0 to 5.4±2.0 mg/g, p<0.05) and a trend towards an increase in total organic acids (7.0±2.4 to 11.8±5.5 mg/g, p=0.12). Indole, a putrefactive product, remained unchanged in the negative group (54.3±25.9 to 56.0±36.2 μg/g, p=0.94) but showed a decreasing trend in the positive group (45.3±32.0 to 26.2±9.2 μg/g, p=0.23). During this period, the intake of dietary fiber changed.

Conclusion: The results suggest that the improvement in gut microbiota diversity in enteral nutrition patients may be related to the proportion of Actinobacteria, potentially influencing patient health through acetic acid and indole. These changes are likely influenced by the type and amount of dietary fiber contained in the liquid diets. By advancing this research, we aim to achieve better enteral nutrition practices focusing on gut microbiota.

Disclosure of Interest: None declared