PP219 - TRIGONA HONEY SUPPLEMENTATION ATTENUATES PREDIABETES-INDUCED MUSCLE STRENGTH DECLINE IN MALE SPRAGUE DAWLEY RATS: A RANDOMIZED PRECLINICAL STUDY

PP219

TRIGONA HONEY SUPPLEMENTATION ATTENUATES PREDIABETES-INDUCED MUSCLE STRENGTH DECLINE IN MALE SPRAGUE DAWLEY RATS: A RANDOMIZED PRECLINICAL STUDY

P. Pitriani^1,*, H. R. D. Ray¹, A. Sulastri², D. Rahmah¹

¹Medicine, ²Nursing, Universitas Pendidikan Indonesia, Bandung, Indonesia

Rationale: Prediabetes is associated with early skeletal muscle dysfunction and elevated sarcopenia risk, driven by chronic inflammation, metabolic dysregulation, and mitochondrial impairment. This study evaluated whether Trigona honey supplementation could attenuate muscle strength decline in a rat model of diet-induced prediabetes.

Methods: Male Sprague-Dawley rats were randomized into four groups: normal diet control (ND), prediabetic control receiving a cholesterol-fat-enriched diet with fructose (CFEDF), and low- or high-dose Trigona honey plus CFEDF. After four weeks, muscle strength was assessed using the four-limb grip strength test. Biomarkers evaluated included myostatin, insulin-like growth factor-1 (IGF-1), AMP-activated protein kinase (AMPK), sirtuin-1 (SIRT1), peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), lipid profile, fasting glucose, and gut microbiome composition.

Results: Trigona honey significantly improved muscle strength vs. CFEDF (reverse hang time and minimum holding impulse, both p<0.001). TNF-α was reduced (p<0.001) while IL-10 (p=0.002), PGC-1α (p<0.001), AMPK (p<0.001), SIRT1 (p<0.001), and IGF-1 (p=0.004) increased. Lipid profiles improved (HDL↑, LDL↓, triglycerides↓, total cholesterol↓; all p≤0.001) alongside reduced fasting glucose (p<0.001). High-dose supplementation increased gut microbiome genus richness (p=0.021) with compositional shifts toward short-chain fatty acid-producing bacteria.

Conclusion: Trigona honey supplementation attenuates prediabetes-associated muscle strength decline by modulating inflammatory signaling, mitochondrial metabolic pathways, and gut microbiome composition, supporting its potential as a functional nutritional strategy for early sarcopenic prevention in metabolic dysfunction.

Disclosure of Interest: None declared