P180 - ASSOCIATION BETWEEN CARDIORESPIRATORY FITNESS AND MITOCHONDRIAL ANTIOXIDANT CAPACITY IN PBMCS: A COMPARISON OF METABOLIC, INFLAMMATORY, AND OXIDATIVE STRESS BIOMARKERS IN SEDENTARY, PHYSICALLY ACTIVE, AND ENDURANCE ATHLETES

P180

"ASSOCIATION BETWEEN CARDIORESPIRATORY FITNESS AND MITOCHONDRIAL ANTIOXIDANT CAPACITY IN PBMCS: A COMPARISON OF METABOLIC, INFLAMMATORY, AND OXIDATIVE STRESS BIOMARKERS IN SEDENTARY, PHYSICALLY ACTIVE, AND ENDURANCE ATHLETES"

O. A. Hernández López^1,2,*, I. Torre Villalvazo³, S. R. Llopis ¹, C. Luna Marco ¹, J. Cacace², A. Hermo Arigibay ², V. M. Víctor Gonzalez ¹

¹Physiology , Valencia University , ²Department of Endocrinology and Nutrition Hospital Universitario Dr. Peset, Foundation for the Promotion of Health and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain, ³Physiology of Nutrition, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City, Mexico

Rationale: What is the relationship of CRF with mitochondrial antioxidant capacity in PBMC and with metabolic, inflammatory and oxidative stress biomarkers in sedentary and physically active individuals?

Methods: This cross-sectional, comparative study involved 49 healthy adults (18–45 years), classified as SED, PA, or EA via IPAQ. It assessed anthropometric, biochemical, hormonal, metabolic, and inflammatory markers, alongside oxidative stress (H2O2, MDA), antioxidant enzyme expression in PBMCs, metabolic flexibility (indirect calorimetry), and CRF (VO2Peak). Statistical tests were used to compare variables between groups and to perform correlation analyses.

Results: PA and EA groups demonstrated greater metabolic flexibility and CRF than the SED group, which exhibited impaired metabolic markers, elevated inflammation and oxidative stress, and reduced antioxidant gene/protein expression. Notably, PA individuals showed a superior antioxidant profile compared to EA, despite similar metabolic benefits.

Conclusion: Low cardiorespiratory fitness (CRF) is associated with systemic inflammation, oxidative stress, insulin resistance, and reduced mitochondrial antioxidant capacity in PBMCs. Higher VO2Peak levels correlate inversely with HOMA-IR, MDA, and TNF-α, suggesting a protective role of elevated CRF against metabolic dysfunction in sedentary individuals.

Disclosure of Interest: None declared