P140 - ASSOCIATION BETWEEN DIETARY INTAKE AND BLOOD CONCENTRATIONS OF VITAMINS A, B6, B9, B12, C, AND E WITH PLASMA EXPRESSION OF MICRORNA-532 IN ADOLESCENTS.

P140

ASSOCIATION BETWEEN DIETARY INTAKE AND BLOOD CONCENTRATIONS OF VITAMINS A, B6, B9, B12, C, AND E WITH PLASMA EXPRESSION OF MICRORNA-532 IN ADOLESCENTS.

H. C. Vidal^1,*, N. E. Delmicon¹, R. M. Fisberg¹, F. M. Sarti², S. E. D. F. Lucena³, M. M. Rogero¹

¹Department of Nutrition, School of Public Health, University of São Paulo, ²School of Arts, Sciences and Humanities, University of São Paulo, São Paulo, SP, ³Department of Statistics and Actuarial Sciences, Federal University of Sergipe, São Cristóvão, SE, Brazil

Rationale: Vitamins are essential regulators, acting as cofactors in metabolism and influencing epigenetic mechanisms via microRNA expression. However, their specific regulatory roles remain unclear; thus, this study investigated the relationship between dietary intake and plasma concentrations of vitamins A, B6, B9, B12, C, and E with plasma expression of microRNA-532 in Brazilian adolescents.

Methods: A cross-sectional study was conducted with a probabilistic sample of 185 adolescents (12-19 years) in São Paulo, Brazil. Dietary data were collected using two non-consecutive 24-hour dietary recalls, and habitual intake was estimated using the Multiple Source Method. Plasma concentrations of vitamins A, B6, C, and E were assessed in heparinized plasma using high-performance liquid chromatography. Vitamin B12 and folate were measured by chemiluminescent immunoassay, with folate analyzed in both serum and erythrocytes. Plasma miR-532 expression was quantified using RT-qPCR and normalized by fold-change. Associations were analyzed using generalized linear models at a 0.05 significance level, adjusting for additional variables.

Results: Dietary intakes of vitamin B1 (p = 0.0007), B6 (p = 0.0149), and A (p = 0.0290) were positively associated with increased plasma expression of miR-532. In contrast, vitamin B2 (p = 0.0025) showed an inverse association, suggesting higher riboflavin intake reduces its expression. Furthermore, plasma vitamin B6 concentration was also positively associated with miR-532 expression (p = 0.0132), independent of dietary intake.

Conclusion: Vitamins B1, B6, and A may upregulate miR-532 expression, while vitamin B2 may have a negative regulatory effect. Notably, plasma vitamin B6 concentration emerged as a potential key modulator, given its association with miR-532 expression in both dietary intake and plasma concentration.

Disclosure of Interest: None declared