LB028 - ASSOCIATION BETWEEN POLYUNSATURATED FATTY ACIDS INTAKE AND PLASMA EXPRESSION OF MICRORNA-376A-3P IN ADULTS.

LB028

ASSOCIATION BETWEEN POLYUNSATURATED FATTY ACIDS INTAKE AND PLASMA EXPRESSION OF MICRORNA-376A-3P IN ADULTS.

N. E. Delmicon^1,*, H. C. Vidal², P. N. Brandão-Lima¹, F. M. Sarti³, R. M. Fisberg¹, S. E. D. F. Lucena⁴, M. M. Rogero¹

¹Department of Nutrition, University of Sao Paulo - School of Public Health, ²Department of Nutrition, ³School of Arts, Sciences and Humanities (EACH), University of Sao Paulo, Sao Paulo, ⁴Departament of Statistics and Actuarial Sciences, Federal University of Sergipe, São Cristóvão, Brazil

Rationale: Dietary patterns rich in omega-6 polyunsaturated fatty acids (PUFAs) that are characterized by high ratio of omega-6 to omega-3 (ω6/ω3) essential fatty acids may promote the synthesis of pro-inflammatory lipid mediators involved in atherogenesis, influencing epigenetic mechanisms, including the regulation of microRNAs that modulate the expression of genes involved in inflammatory responses. This study aims to investigate the association between plasma expression of miR-376a and PUFA intake in adults participating in a subsample of a population-based study.

Methods: This cross-sectional study included 188 adults (aged 20 to 59 years) residing in São Paulo city, Brazil. PUFA intake was estimated using two 24-hour dietary recalls, and the ω6/ω3 ratio was calculated as the ratio of omega-6 to omega-3 intake. Dyslipidemias were assessed based on alterations in at least one of the following serum markers: low HDL-c, high LDL-c, or elevated triacylglycerols, determined by enzymatic colorimetric assays. Plasma expression of miR-376a was quantified using RT-qPCR, and predicted targets were investigated using the miRDB tool. Finally, associations between plasma expression of miR-376a and the other variables were tested using generalized linear models, with a significance level set at 0.05.

Results: Plasma expression of miR-376a was positively associated with omega-6 intake (p = 0.024) and the ω6/ω3 ratio (p = 0.011). Additionally, positive associations were observed between miR-376a and serum concentrations of HDL-c (p = 0.009), LDL-c (p = 0.001), and triacylglycerols (p = 0.009). In silico analyses indicated that NFKBIZ and NFAM1, genes involved in inflammatory responses, are potential targets of miR-376a.

Conclusion: These findings suggest that plasma expression of miR-376a may be modulated by dietary intake of omega-6 PUFAs and is associated with lipid profile.

Disclosure of Interest: None declared