P824 - NETWORK-BASED INVESTIGATION OF MIRNA AND MICROBIOME INTERACTIONS IN PARKINSON’S AND HUNTINGTON’S DISEASE: A SYSTEMS BIOLOGY APPROACH

P824

NETWORK-BASED INVESTIGATION OF MIRNA AND MICROBIOME INTERACTIONS IN PARKINSON’S AND HUNTINGTON’S DISEASE: A SYSTEMS BIOLOGY APPROACH

I. M. Durasi^1,*

¹Molecular Biology and Genetics, Istanbul Health and Technology University, Istanbul, Türkiye

Rationale: Parkinson’s and Huntington’s diseases are complex neurodegenerative disorders involving both genetic dysregulation and host–microbiome interactions. While miRNAs are key post-transcriptional regulators in brain homeostasis, gut microbiota also influence host gene expression, particularly through immune and metabolic pathways. This study aims to identify disease-specific regulatory modules by integrating miRNA, transcription factors (TFs), gene expression, and microbiome–host interactions.

Methods: Transcriptomic and miRNA-seq data from prefrontal cortex samples (GSE64810, GSE68719, GSE64977, GSE72962) were analyzed to identify differentially expressed genes (DEGs) and miRNAs (DEmiRs). A directed regulatory network was constructed using validated interactions from miRTarBase, TransmiR, SIGNOR, and GutMGene. Disease-specific subnetworks were extracted using breadth-first search (BFS), followed by Louvain clustering and KEGG enrichment analysis to define functional modules.

Results: Integrated networks revealed distinct miRNA–TF–gene–microbiome modules enriched in apoptosis, mitochondrial dysfunction, and neuroinflammation. miRNAs such as miR-146a and miR-9 were central regulators in cascades linked to immune signaling. Literature-based microbial taxa including Prevotella and Akkermansia interacted with host genes related to synaptic transmission and cytokine regulation, suggesting microbiota-driven modulation of neurodegenerative pathways.

Image:

Conclusion: This multi-omics systems biology approach uncovers key regulatory axes linking miRNAs, gene expression, and microbiome–host crosstalk in neurodegenerative diseases. Findings may guide microbiome-targeted nutritional or therapeutic strategies for brain health and clinical management of Parkinson’s and Huntington’s disease.

Disclosure of Interest: None declared