P844 - CHANGES OF INTESTINAL FLORA COMPOSITION IN HUMAN FLORA-ASSOCIATED RATS BY HIGH FAT DIET

P844

CHANGES OF INTESTINAL FLORA COMPOSITION IN HUMAN FLORA-ASSOCIATED RATS BY HIGH FAT DIET

D. Chen^1,*, B. Zhang¹, O. Wang¹, X. Zhang², J. Huang¹

¹National Institute for Nutrition and Health，Chinese Center for Disease Control and Prevention, ²The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/ National Center of Gerontology of National Health Commission, P.R. China, Beijing, China

Rationale: To study the changes in fat accumulation and gut microbiota under high-fat diet feeding using different human gut microbiota rat (HFA rat) models.

Methods: Fourteen volunteers aged 45-60 were recruited from Yueyang City, Hunan Province. Among them, seven males were included in the dyslipidemia group (DH), defined as TG>1.7 mmol/L, and the remaining seven males with TG<1.7 mmol/L were included in the normal blood lipid group (EH). Constructing HFA rat model using sterile SD rats. Sterile rats were divided into three groups (n=6), namely DH, EH, and non colonized group (FH). After colonization, a 9-week high-fat diet feeding experiment was conducted. After the experiment, the liver,  perirenal, subcutaneous, and brown adipose tissues of the mice were weighed to indicate fat accumulation. Measurement of blood lipid indicators, HE staining, and high-throughput sequencing analysis of changes in the gut microbiota of rats.

Results: Compared with the EH group, there were no significant differences in body weight, perirenal, subcutaneous and brown adipose tissue mass, and blood lipids in the DH group after the experiment. However, both the liver HE staining DH group and FH group showed more severe steatosis and fat vacuoles. After 9 weeks of high-fat diet, the ratio of Firmicutes and Bacteroidetes in the DH group was significantly higher than that in the EH group. Under a high-fat diet, the DH group is more likely to cause disturbances in the gut microbiota, with the relative abundance of beneficial bacteria being suppressed and the abundance of pathogenic bacteria increasing.

Conclusion: The colonization of gut microbiota in volunteers with different blood lipid levels under a high-fat diet is related to changes in body weight and fat accumulation in rats. The species composition and abundance of microbiota in different populations have undergone significant changes.

Disclosure of Interest: None declared