PW07 - PROTECTIVE EFFECT OF MILK BIOFORTIFIED WITH SELENIUMMETHIONINE ON THE ALLERGENIC IMMUNE RESPONSE
PW07
PROTECTIVE EFFECT OF MILK BIOFORTIFIED WITH SELENIUMMETHIONINE ON THE ALLERGENIC IMMUNE RESPONSE
A. M. Navarro1,*, T. R. Reis1, J. E. Oliveira2, W. M. Rios2, A. S. Netto3, D. C. Sartori2
1Department of Health Sciences , 2Laboratory of Immunoregulation of Metabolic Diseases, Department of Biochemistry and Immunology, Ribeirão Preto Medical School - University of Sao Paulo, Ribeirão Preto, 3Faculty of Animal Science and Food Engineering - University of Sao Paulo, Pirassununga, Brazil
Rationale: Cow's milk protein allergy (CMPA) is one of the most prevalent forms of food allergy and constitutes a growing public health problem given the complete exclusion of milk from the diet as a therapeutic approach. Thus, alternatives that seek to attenuate the allergic response without eliminating the food from the diet, such as the biofortification of milk with selenium (Se), seem promising. Se has anti-inflammatory and antioxidant properties, promoting the inhibition of mast cell degranulation. Thus the objective of this study is to evaluate the immunomodulatory potential of freeze-dried milk biofortified with seleniummethionine (SeMet) on the allergic response to cow's milk protein.
Methods: Initially, for the production of biofortified milk, the cows were fed with a diet supplemented with SeMet (2.5 mgSe/kg of diet) and without SeMet supplementation. After this period, the milk produced by the cows was freeze-dried, obtaining a proportion of 0.18 μgSe/g of biofortified milk powder and 0.05 μgSe/g of non-biofortified milk powder. Milk solutions at 50% were prepared to perform the Allergen Experiment. For this, BALB/c mice were selected, sensitized with β-lactoglobulin (BLG) associated with aluminum hydroxide or alum, via intraperitoneal route. The animals were divided into three Experimental Groups: Water Vehicle Group (WVG, n=6), Non-Biofortified Milk Group (NBMG, n=6) and Biofortified Milk Group (BMG, n=6). Each group received daily by gavage 0.2 mL of the solution corresponding to the study group (water or non-biofortified milk or biofortified milk), for 14 days. On the seventh and fourteenth days, serum and jejunoileal lymph nodes were collected for analysis of cell populations, performed by flow cytometry for the following markers: B lymphocytes: CD19⁺ cells; Regulatory T cells (Treg): CD3⁺, CD4⁺ Foxp3⁺; Th2 cells: CD3⁺, CD4⁺, GATA3⁺.
Results: It was observed that the percentage of CD19⁺ was slightly higher in the BMG group compared to the WVG group and NBMG (p<0.05). However, no significant differences were observed in the absolute number of CD19⁺ cells between the groups. Regarding Th2 cells, there was a tendency for an increase in the percentage of Th2 cells in the BMG group compared to the others, although not significant. The absolute number of Th2 cells also did not show significant differences between the experimental groups. The B cell data indicate that there was no statistically significant variation in either the percentage or the absolute number of CD3⁺ CD4⁺ Foxp3⁺ cells between the groups studied.
Conclusion: Biofortification with SeMet has shown promise for the nutritional enrichment of selenium in cow's milk, increasing selenium bioavailability and enhancing its immunomodulatory action. Flow cytometry data demonstrated that milk biofortified with SeMet was able to increase the percentage of CD19⁺ B lymphocytes in the jejunoileal lymph nodes, suggesting its possible immunomodulatory effect on the humoral response, enabling the use of milk biofortified with SeMet as a future complementary strategy in dietary interventions for the nutritional management of CMPA.
References: CRONIN, C.; RAMESH, Y.; DE PIERI, C.; VELASCO, R.; TRUJILLO, J. ‘Early Introduction’ of Cow’s Milk for Children with IgE-Mediated Cow’s Milk Protein Allergy: A Review of Current and Emerging Approaches for CMPA Management. Nutrients, v. 15, p. 1397, 2023. ZHANG, Y.; CARTLÂNDIA, S. P.; HENRIQUEZ, R.; PATEL, S.; GAMMELGAARD, B.; FLOUDA, K.; HAWKINS, C. L.; RAYNER, B. S. A suplementação de selenometionina reduz a carga das lesões, melhora a função dos vasos e modula a resposta inflamatória no contexto da aterosclerose. *Redox Biology*, v. 29, p. 101409, 2020.
FAPESP Process no. 2021/07131-0
Disclosure of Interest: None declared