P673 - MATERNAL MALNUTRITION DURING PREGNANCY INCREASES SUSCEPTIBILITY TO INFECTIONS IN ADULT OFFSPRING BY COMPROMISING THE PHAGOCYTIC ACTIVITY OF ALVEOLAR MACROPHAGES

P673

MATERNAL MALNUTRITION DURING PREGNANCY INCREASES SUSCEPTIBILITY TO INFECTIONS IN ADULT OFFSPRING BY COMPROMISING THE PHAGOCYTIC ACTIVITY OF ALVEOLAR MACROPHAGES

G. A. Azevedo¹, M. E. B. Padua¹, N. G. S. Negreiros¹, B. K. Lippi¹, R. R. Ferreira^1,2, M. A. Landgraf³, R. G. Landgraf^1,*

¹Pharmaceutical Sciences, Federal University of São Paulo, Diadema, ²University of São Paulo, São Paulo, ³Universidade Paulista, Santos, Brazil

Rationale: Maternal food restriction during pregnancy induces morphological and metabolic fetal adaptations and predisposes offspring to metabolic diseases and defective inflammatory responses. This study aimed to evaluate the influence of maternal malnutrition during pregnancy on the development of sepsis in adult Offspring.

Methods: Pregnant female Wistar rats were subjected to 50% calorie-protein food restriction throughout gestation. At 12 weeks of age, male offspring rats were subjected to cecal ligation and puncture or a sham operation. Six hours later, the animals were euthanized for evaluation of temperature, glycemia, creatinine and urea (urine), cytokines (in serum and lung tissue), and the phagocytic activity of alveolar macrophages incubated with Saccharomyces cerevisiae. (CEUA-UNIFESP 2268240919)

Results: Malnutrition during gestation caused a reduction in fetal birth weight (LBW) compared to the offspring of the control group (normal birth weight - NBW). Hypothermia, hyperglycemia, and elevated levels of creatinine and urea were also observed in both groups subjected to the sepsis protocol. Serum levels of IL-6, IL-1β, IL-10, TNF-α, and MIP-2 were higher in the LBW group than in the NBW group. The colony-forming unit count in the peritoneal fluid was significantly higher in the LBW group than in the NBW group. The LBW group showed defective phagocytic activity, as the number of yeasts phagocytosed by alveolar macrophages was lower than in the NBW group.

Conclusion: Low birth weight induced by maternal malnutrition during pregnancy impairs the phagocytic activity of alveolar macrophages and may compromise the immune response, increasing susceptibility to infections.

Disclosure of Interest: None declared