P803 - EFFECT OF ADVANCED MATERNAL AGE ON PLACENTAL SIGNALLING OF PAI-1, LEPTIN AND VISFATIN

P803

EFFECT OF ADVANCED MATERNAL AGE ON PLACENTAL SIGNALLING OF PAI-1, LEPTIN AND VISFATIN

J. J. Ochoa^1,2,3,*, J. M. Toledano^1,2, M. Puche-Juarez^1,2, J. Moreno-Fernandez^1,2,4, R. Ruiz-Sanchez¹, C. de Paco-Matallana^5,6, J. Sanchez-Moreno⁵, M. P. Carrillo⁷, J. Diaz-Castro^1,2,4

¹Physiology, University of Granada, ²Institute of Nutrition and Food Technology “José Mataix Verdú”, Biochemical Research Centre, ³Investigacion Biosanitaria (IBS), , ⁴Instituto de Investigacion Biosanitaria (IBS), Granada, ⁵Obstetrics and Gynecology, ‘Virgen de la Arrixaca’ University Hospital, ⁶Institute for Biomedical Research of Murcia, Murcia, ⁷Obstetrics & Gynaecology, Virgen de las Nieves University, Granada, Granada, Spain

Rationale: Advanced maternal age (AMA) refers to nulliparous mothers aged 35 years or older and has been linked to an elevated risk of obstetrical issues. Age affects the adipokine profile and adipokines imbalance during gestation has been linked to several impairments and comorbidities in progeny and in this process, placenta play an important role. The aim of this study is to elucidate the influence of advanced maternal age on placental PAI-1, leptin and visfatin signalling.

Methods: A cohort of 200 nulliparous pregnant women were recruited from two hospital; Hospital Materno-Infantil Virgen de las Nieves (Granada, Spain) and Hospital Universitario Virgen de la Arrixaca (Murcia, Spain). A sample of 6 × 6 × 6 cm placental cotyledons was collected, excluding placental membranas and proteins were isolated from placenta samples by mechanical homogenization in tissue protein extraction reagent (T-PER). The adipokines studied were determined using the Bio-Plex ProHuman Diabetes Assays.

Results: PAI-1 concentrations were lower in the placenta of the AMA group than in the control group, with statistically significant differences (p<0.05). Similarly, leptin concentrations in the placenta of the AMA group were lower than in the control group (p<0.05). In contrast, the results showed higher visfatin concentrations in the AMA group than in the control group, with statistically significant differences (p<0.05).

Conclusion: The results show altered placental PAI-1, leptin, and visfatin signaling in older mothers. The involvement of these adipokines in metabolic regulation, energy homeostasis, fibrinolysis, and endothelial function, all of which are critical for the successful outcome of pregnancy and fetal development, could help explain the higher prevalence of diabetes, preeclampsia, and other obstetric abnormalities observed in these mothers.

Disclosure of Interest: None declared