P865 - ALLELIC DISTRIBUTION OF METABOLIC DISEASE-RELATED GENES IN CZECH AND VIETNAMESE POPULATIONS: A COMPARATIVE APPROACH

P865

ALLELIC DISTRIBUTION OF METABOLIC DISEASE-RELATED GENES IN CZECH AND VIETNAMESE POPULATIONS: A COMPARATIVE APPROACH

K. H. Pham^1,*, J. Hubáček^2,3

¹Thomayer University Hospital, ²Experimental Medicine Centre, Institute of Clinical and Experimental Medicine, ³3rd Department of Internal Medicine, First Faculty of Medicine, Charles University, Prague, Czech Republic

Rationale: Civilization diseases, such as type 2 diabetes mellitus (T2DM), obesity, and dyslipidemia, not only increase illness and mortality risks but also impose significant economic and healthcare burdens. These diseases affect both industrialized countries and developing countries. Certain genetic variants increase susceptibility to these diseases, with prevalence varying across populations. This study aimed to investigate the allelic distribution of selected nutrigenetically relevant genes in the Czech and Vietnamese populations.

Methods: Samples from 94 Vietnamese participants and 2,859 Czech individuals (2,559 from the Czech post-MONICA project and 300 volunteers from the southern region of the Czech Republic) were analyzed using the PCR-RFLP method or the SNP Genotyping Assays on a Real-Time PCR System.

Results: Significant differences in allele frequencies were observed between the two populations for most, but not all, of the single nucleotide polymorphisms (SNPs) examined. Notably, the prevalence of risk alleles in the studied polymorphisms was generally lower in the Vietnamese population compared to the Czech population, particularly within the FTO (rs17817449, associated with obesity risk, P < 0.0001), TCF7L2 (rs7903146, associated with T2DM, P < 0.0001), and ADH1B (rs1229984, related to alcohol consumption, P < 0.0001). The MCM6/LCT cluster (rs4988235), linked to lactase persistence, was absent in the Vietnamese cohort. A minor genotype difference was detected for one HFE polymorphism (rs1799945, P = 0.01), though no significant variation was observed for rs1800562. The genotype frequencies of the MC4R and APOE genes were nearly identical in both populations.

Conclusion: The results highlight some major differences in genetic background between populations and will contribute to a deeper understanding of these variants in a less studied context.

Disclosure of Interest: None declared