P838 - EFFECT OF 12-WEEK CALORIE RESTRICTION COMBINED WITH PROBIOTIC SUPPLEMENTATION ON IMMUNOLOGICAL PARAMETERS IN MEN WITH OBESITY

P838

EFFECT OF 12-WEEK CALORIE RESTRICTION COMBINED WITH PROBIOTIC SUPPLEMENTATION ON IMMUNOLOGICAL PARAMETERS IN MEN WITH OBESITY

A. C. O. Magalhães^1,*, C. G. Marques¹, M. V. L. D. S. Quaresma², G. A. Lucin¹, C. B. F. Ferracini², F. B. A. Carrilho², G. L. Mendes¹, F. P. Nakamoto³, R. V. Thomatieli-Santos¹

¹Psychobiology, Federal University of São Paulo, ²Nutrition, ³Medicine, São Camilo University Center, São Paulo, Brazil

Rationale: Obesity is linked to chronic low-grade inflammation, contributing to metabolic and immune dysfunction. While caloric restriction (CR) may have immunomodulatory effects, the potential additive benefits of probiotic supplementation remain unclear. It is also unknown whether the magnitude of weight loss influences these outcomes.

Methods: This 12-week randomized, double-blind, placebo-controlled clinical trial involved adult men with BMI 30–39.9 kg/m². Participants followed calorie restriction (30% of total energy expenditure) plus a probiotic (CRPRO) [8 billion CFU of L. acidophilus, L. rhamnosus, L. paracasei, and B. lactis] or placebo (CRPLA). Immunological markers (TNF-α, IL-6, IL-10) and body composition were assessed pre- and post- intervention. We applied repeated measures ANOVA and adjusted for the magnitude of weight loss (<5% vs ³5%).

Results: Thirty-three participants were evaluated (36.5±4.43 years; 34.5±2.39 kg/m²; 37.2±5.48 kg of fat mass), of whom 17 were in the CRPLA group (36.4±3.94 years; 35.1±2.37 kg/m²; 38.5±4.70 kg of fat mass) and 16 in the CRPRO group (34.6±4.87 years; 33.9±2.33 kg/m²; 35.9±6.08 kg of fat mass). At baseline, IL-6, TNF-α, and IL-10 levels were 20.02±7.11 vs 23.06±7.94; 1.53±1.29 vs 1.37±1.21; and 7.27±6.98 vs 9.06±10.73 for the CRPLA and CRPRO groups, respectively, with no significant differences between groups (all p-values >0.05). The p-values for IL-6, TNF-α, and IL-10 were, respectively, p= 0.173; p= 0.513; and p=0.556, indicating that CR alone did not promote changes in IL-6, TNF-α, or IL-10, regardless of probiotic supplementation (p=0.425; p=0.681; p=0.234) or the magnitude of weight loss (p=0.095; p=0.450; p=0.276).

Conclusion: These findings suggest that, contrary to our hypothesis, CR alone, regardless of probiotic supplementation or weight loss magnitude (<5% vs ³5%), did not promote a reduction in inflammatory mediators.

Disclosure of Interest: None declared