P077 - AN HERBAL FORMULA ENRICHED WITH CERVUS ELAPHUS AND GLYCINE MAX AMELIORATES OVARIECTOMY-INDUCED BONE LOSS IN C57BL/6J MICE

P077

AN HERBAL FORMULA ENRICHED WITH CERVUS ELAPHUS AND GLYCINE MAX AMELIORATES OVARIECTOMY-INDUCED BONE LOSS IN C57BL/6J MICE

E.-J. Lee^1,* on behalf of This study was conducted with contributions from Dr. Doyoung Kwon (Department of Korean Rehabilitation Medicine, College of Korean Medicine, Daejeon University) and Researcher Min-young Chae (Institute of Bioscience & Integrative Medicine)

¹Department of Korean Rehabilitation Medicine, College of Korean Medicine, Daejeon University, Daejeon, Korea, Republic Of

Rationale: Osteoporosis increases fracture risk due to reduced bone mineral density (BMD), impairing quality of life in aging populations. An herbal formula (CG), primarily composed of Cervus elaphus sibiricus and Glycine max (L.) Merrill—two key ingredients known to support bone metabolism—was evaluated for its effects in an ovariectomized (OVX) osteoporotic mouse model.

Methods: OVX was performed on 7-week-old female C57BL/6J mice, followed by a 12-week induction. Mice then received a 12-week treatment with CG (250 or 500 mg/kg) or alendronate (ALD) 5 mg/kg. BMD and bone microarchitecture were assessed using micro-computed tomography (µCT), and bone remodeling markers were evaluated by western blotting and RT-PCR. One-way ANOVA with Dunnett’s test was used (p < 0.05).

Results: The OVX group showed an 18.8% reduction in BMD compared to the sham group. CG (500 mg/kg) significantly increased BMD and improved BV/TV, Tb.Th, Tb.N, and Tb.Sp, as assessed by µCT (Figures A–F). CG downregulated osteoclast markers TRAP, NFATc1, and Tnfsf11, and upregulated osteoblast markers RUNX2, ALP, osteocalcin, Bmp2, and Tnfrsf11b. Serum BALP, CTX-I, and calcium levels were also significantly modulated.

Image:

Conclusion: The CG formula increased BMD and promoted bone remodeling in OVX-induced osteoporotic mice. These findings support its therapeutic potential for postmenopausal osteoporosis, warranting further clinical validation.

References: 1.Chandran M et al. Prevalence of osteoporosis and incidence of related fractures in developed economies in the Asia Pacific region: a systematic review. Osteoporos Int. 2023;34(6):1037-1053.

2.Baek DC et al. A Mixture of Cervus elaphus sibiricus and Glycine max (L.) Merrill Inhibits Ovariectomy-Induced Bone Loss Via Regulation of Osteogenic Molecules in a Mouse Model. Int J Mol Sci. 2023;24(5):4876.

Disclosure of Interest: None declared