P500 - FECAL FUNGS ACTIVATE HEPATIC LIPID ASSOCIATED MACROPHAGES VIA CLRS AND SECRETE GPNMB IN PARENTERAL NUTRITION ASSOCIATED LIVER DISEASE

P500

FECAL FUNGS ACTIVATE HEPATIC LIPID ASSOCIATED MACROPHAGES VIA CLRS AND SECRETE GPNMB IN PARENTERAL NUTRITION ASSOCIATED LIVER DISEASE

G. Maitiabula¹, L. Zhang¹, X. Gao¹, X. Wang ^1,*

¹Department of General Surgery, Nanjing Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China

Rationale: Intestinal failure (IF) is a common and potentially fatal organ injury in clinical practice. Parenteral nutrition (PN)-associated liver disease (PNALD) is the serious complication of long-term use of PN and has been identified as a negative predictor of survival in IF patients

Methods: We intend to further elucidate the roles and molecular mechanisms of mannan generating GPNMB production by LAMs via CLRs by ITS and single fungal colonization.

Results: ①The abundance of intestinal fungi in PNALD patients was changed, Candida tropicalis and mannan, the product of Candida tropicalis, was increased in PNALD patients; ②The mannan-acting receptors CLRs were increased in liver of PNALD mice. ③ Lipid-associated macrophages (LAMs) related genes and GPNMB were also increased in livers of PNALD mice and patients.

Conclusion: Intestinal fungal changes and liver macrophage reprogramming are the key to the development of PNALD. Mannan, derived from intestinal Candida tropicalis, binds to CLRs to activate LAMs, and participate development of PNALD

Disclosure of Interest: None declared