PD255 - UNILATERAL LIGATION OF COMMON CAROTID ARTERY WORSENS SURVIVAL IN A MURINE GUT ISCHEMIA REPERFUSION MODEL

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PD255

UNILATERAL LIGATION OF COMMON CAROTID ARTERY WORSENS SURVIVAL IN A MURINE GUT ISCHEMIA REPERFUSION MODEL

H. Takayama1,2,*, K. Fukatsu1, T. Narita1,3, M. Noguchi1, S. Tsuihiji1, S. Murakoshi1,4

1Surgical Center, The University of Tokyo Hospital, 2Nutrition, St. Luke's International Hospital, 3Gastrointestinal Surgery, The University of Tokyo Hospital, Tokyo, 4Health & Social Work, The Kanagawa University of Human Services, Kanagawa, Japan

 

Rationale: Our recent prospective observational study demonstrated that the reduction of gut and/or carotid artery blood flow at early time points after ICU admission is associated with poor prognosis.  However, whether reduced brain blood flow itself worsens the outcome of critically ill or injured patients or is an accompanying phenomenon remains unclear.  In this study, we examined the influences of unilateral ligation of the common carotid artery before gut ischemia-reperfusion on survival in mice.

Methods: Five-week-old male ICR mice were divided into two groups: a CCA ligation group (Ligation group: n=12) and a non-ligation group (Sham group: n=10). All mice were acclimated for 7 days under eating Chow and drinking water ad libitum. After the period, the Ligation group mice underwent right CCA ligation, whereas the Sham group mice were only exposed to their CCA without ligation. Subsequently, all mice received the superior mesenteric artery (SMA) occlusion for 15 minutes under temperature control. Survival after SMA reperfusion was observed for 120 hours. Survival times were statistically analyzed using the Log-rank test, with a significance level of less than 5%.

Results: Survival curves are shown in Figure 1. The survival time was significantly shorter in the Ligation group as compared to the Sham group (Log-rank test: p=0.0173). 

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Conclusion: Reduced brain blood flow may be a mechanism underlying the poor prognosis of severe ICU patients.  Measurement of a carotid artery blood flow could be a new evaluation method for ICU patients’ prognosis.  Moreover, appropriate control of brain blood flow might improve their survival.  We are now investigating the inflammatory mediators’ profile and organ injury in this model to clarify the possible linkage between brain blood flow and prognosis of severe injury.

Disclosure of Interest: None declared